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hard shell pcr plates  (Bio-Rad)


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    Bio-Rad hard shell pcr plates
    Hard Shell Pcr Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+plate/Hard-Shell+96-Well+PCR+Plates/pmc13092596-31-0-4
    Average 96 stars, based on 1411 article reviews
    hard shell pcr plates - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Perl script bigsam_to_wig_mm10_wcigar2.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script scale_wiggle_rpm.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script macs_strand_rand_sam.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Python script create_bkg_seq.py This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR CFX Manager Bio-Rad v3.1 Prism GraphPad v9.5.0 Other Forma Series II Water-Jacketed CO 2 Incubator Thermo Fisher Scientific 3110 Vibra-Cell 130-W probe-tip sonicator Sonics VCX 130 2200 TapeStation System Agilent G2964AA High Sensitivity D1000 ScreenTape Agilent 5067–5584 High Sensitivity D1000 Reagents Agilent 5067–5585 NextSeq1000 sequencing platform Illumina 20038898 100-cycle NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit Illumina 20100987 Hard-Shell 96-Well PCR Plates Bio-Rad HSP9601 Microseal ’B’ PCR Plate Sealing Film Bio-Rad MSB1001 C1000 Touch Thermal Cycler Bio-Rad 1851196 CFX96 Real-Time System Bio-Rad 1845097 RNaseZap RNA Decontamination Solution Thermo Fisher Scientific AM9780 mESC Growth Medium Reagent Final concentration Amount DMEM (90 mL removed from 500-mL bottle before adding other reagents directly to this bottle) N/A 410 mL ESC-qualified fetal bovine serum (100%) 15% 75 mL Non-essential amino acids (10 mM) 0.1 mM 5 mL Penicillin-streptomycin (10,000 U/mL) 100 U/mL 5 mL L-glutamine (200 mM) 2 mM 5 mL β-mercaptoethanol (100 mM) 0.1 mM 500 μL LIF-conditioned medium (or recombinant LIF obtained commercially, e.g., Gibco, A35934) 1:500 1 mL Total N/A 501.5 mL Store at 4 ◦ C for up to 3 weeks. ..

    Polymerase Chain Reaction:

    Article Title: Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Perl script bigsam_to_wig_mm10_wcigar2.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script scale_wiggle_rpm.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script macs_strand_rand_sam.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Python script create_bkg_seq.py This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR CFX Manager Bio-Rad v3.1 Prism GraphPad v9.5.0 Other Forma Series II Water-Jacketed CO 2 Incubator Thermo Fisher Scientific 3110 Vibra-Cell 130-W probe-tip sonicator Sonics VCX 130 2200 TapeStation System Agilent G2964AA High Sensitivity D1000 ScreenTape Agilent 5067–5584 High Sensitivity D1000 Reagents Agilent 5067–5585 NextSeq1000 sequencing platform Illumina 20038898 100-cycle NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit Illumina 20100987 Hard-Shell 96-Well PCR Plates Bio-Rad HSP9601 Microseal ’B’ PCR Plate Sealing Film Bio-Rad MSB1001 C1000 Touch Thermal Cycler Bio-Rad 1851196 CFX96 Real-Time System Bio-Rad 1845097 RNaseZap RNA Decontamination Solution Thermo Fisher Scientific AM9780 mESC Growth Medium Reagent Final concentration Amount DMEM (90 mL removed from 500-mL bottle before adding other reagents directly to this bottle) N/A 410 mL ESC-qualified fetal bovine serum (100%) 15% 75 mL Non-essential amino acids (10 mM) 0.1 mM 5 mL Penicillin-streptomycin (10,000 U/mL) 100 U/mL 5 mL L-glutamine (200 mM) 2 mM 5 mL β-mercaptoethanol (100 mM) 0.1 mM 500 μL LIF-conditioned medium (or recombinant LIF obtained commercially, e.g., Gibco, A35934) 1:500 1 mL Total N/A 501.5 mL Store at 4 ◦ C for up to 3 weeks. ..

    Article Title: Age-related decline of platelet-derived growth factor receptor alpha in mesenchyme surrounding meibomian gland leads to meibomian gland dysfunction.
    Article Snippet: The meibomian glands (MGs) in the eyelids produce oil to prevent tear evaporation, a function that declines with age.. The mechanisms underlying MGs homeostasis and the pathogenesis leading to age-related meibomian gland dysfunction (ARMGD) remain largely unexplored.. MGs contain secretory acini enveloped by a substantial mesenchyme.

    Article Title: Enrichment of Hypermethylated DNA on Chip for Cervical Cancer Detection
    Article Snippet: .. Gravity flow columns, Experion DNA chips, Microseal ‘B’ PCR Plate Sealing Film, SsoAdvanced Universal SYBR Green Supermix and ddPCR 96-well plates were obtained from BioRad. .. NaCl, β-mercaptoethanol, dimethyl sulfoxide ≥99.7% (DMSO), EDTA, MgCl2 ≥ 98.0% (MgCl2), LB Broth, lysozyme from chicken egg white (≥90% lysozyme), phenylmethanesulfonyl fluoride (PMSF), ribonuclease A from bovine pancreas (RNAse), deoxyribonuclease I from bovine pancreas (DNAse), kanamycin sulfate from Streptomyces kanamyceticus, nickel(II) chloride hexahydrate (NiCl2), Nα,Nα-bis(carboxymethyl)-l-lysine hydrate ≥ 97.0%, sodium carbonate, phosphate-buffered saline (PBS) tablets for 10 mm solution, triethylamine, ammonium hydroxide solution 25%, fluorescein, H2SO4 95– 97%, 0.2 μm membrane filter, GelRed Nucleic Acid Stain, and sodium dodecyl sulfate ≥99% (SDS) were purchased from Sigma–Aldrich.

    Article Title: Quantifying the influence of salinity and density on environmental DNA shedding and decay rates from turtles
    Article Snippet: The Bio-Rad QX200 droplet generator partitioned each reaction mixture into droplets by combining 20 μl of the reaction mixture with 70 μl of droplet generation oil, resulting in a total droplet volume of 40 μl. .. The emulsified samples were transferred to a 96-well PCR plate, heat-sealed with foil and subjected to the following thermal cycling protocol: an initial denaturation at 95°C for 5 min, followed by 40 cycles of denaturation at 95°C for 10 s and an annealing and extension step at 60°C for 30 s. After thermal cycling, the PCR plate was then transferred to the QX200 droplet reader (Bio-Rad, USA) to measure the TaqMan fluorescence of each droplet in each well. ..

    Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability
    Article Snippet: .. Superscript III enzyme (Thermo Fisher Scientific, Cat. No. 18080044) RNase H (New England Biolabs, Cat. No. M0297S) RNase If (New England Biolabs, Cat. No. M0243S) D1000 Screen Tape and Sample Buffer (Agilent, Cat. No. 5067-5582, 5067-5583) NEBNext Library Quant Kit for Illumina (NEB, Cat. No. E7630S) AmpureXP Purification Beads (Beckman Coulter, Cat. No. A63880) Illumina MiSeq Reagent Kit v2 (Illumina, Cat. No. MS-103-1001) PhiX Control V3 (Illumina, Cat. No. FC-110-3001) TapeStation System (Agilent, Model No. 4200) PCR plate (BIO-RAD, Cat. No. MLL9601) Adhesive PCR plate seal (BIO-RAD, Cat. No. MSB1001) Real-Time PCR (BIO-RAD, Model No. CFX Opus 96) MiSeq (Illumina, Model No. SY-410-1003) .. The library specific reverse transcription primers carry a target RNA binding region, 6-nucleotide homemade barcodes, a 4-nucleotide anchor, 10-nucleotide UMI , another 4-nucleotide anchor and part of the Illumina i7 adapter ( ).

    Article Title: A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols from Adhesive Samplers
    Article Snippet: .. The adhesive films used as ‘Sticky Samplers’ were Microseal ‘B’ PCR Plate Sealing Films (Bio-Rad Laboratories, USA; catalogue no. MSB1001B). ..

    Article Title: Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactions
    Article Snippet: Plasmid assembly of toehold switches and truncated targets was automated using a Hamilton Microlab NGS STAR platform, with methods encompassing molecular cloning [polymerase chain reaction (PCR) amplification, Gibson assembly, chemical transformation] and plasmid purification. .. Template DNA was dispensed from IDT V-bottom storage plates (Corning P-96–450V-B) into a skirted PCR plate (Bio-Rad HSP9601). .. The robotically assembled common enzyme mix consisted of 2.5 μl of ultrapure DNase/RNase-free DI water (Thermo Scientific A57775), 0.5 μl of each 10 μM primer (Integrated DNA Technologies, see ), and 5 μl of Q5 2× Master Mix (New England Biolabs M0492L) for each reaction, assembled in a 1.7-ml tube (Genesee Scientific 22–281).

    Article Title: Discovery of tankyrase scaffolding inhibitor specifically targeting the ARC4 peptide binding domain.
    Article Snippet: .. Each condition was studied in four replicates of 20 μL and measured in a sealed MultiplateTM 96-Well PCR plate (MLL9601, Bio-Rad) with the use of a CFX96 Real-Time PCR Detection System (Bio-Rad). .. During the experiment, the temperature was gradually raised from 20 ◦ C to 95 ◦ C (1 ◦ C increase per minute) with measurements performed at intervals of 1 min. After the experiment, data were normalized and analyzed with GraphPad Prism 10 by using a nonlinear regression analysis (Boltzmann sigmoid equation).

    Concentration Assay:

    Article Title: Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Perl script bigsam_to_wig_mm10_wcigar2.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script scale_wiggle_rpm.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script macs_strand_rand_sam.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Python script create_bkg_seq.py This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR CFX Manager Bio-Rad v3.1 Prism GraphPad v9.5.0 Other Forma Series II Water-Jacketed CO 2 Incubator Thermo Fisher Scientific 3110 Vibra-Cell 130-W probe-tip sonicator Sonics VCX 130 2200 TapeStation System Agilent G2964AA High Sensitivity D1000 ScreenTape Agilent 5067–5584 High Sensitivity D1000 Reagents Agilent 5067–5585 NextSeq1000 sequencing platform Illumina 20038898 100-cycle NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit Illumina 20100987 Hard-Shell 96-Well PCR Plates Bio-Rad HSP9601 Microseal ’B’ PCR Plate Sealing Film Bio-Rad MSB1001 C1000 Touch Thermal Cycler Bio-Rad 1851196 CFX96 Real-Time System Bio-Rad 1845097 RNaseZap RNA Decontamination Solution Thermo Fisher Scientific AM9780 mESC Growth Medium Reagent Final concentration Amount DMEM (90 mL removed from 500-mL bottle before adding other reagents directly to this bottle) N/A 410 mL ESC-qualified fetal bovine serum (100%) 15% 75 mL Non-essential amino acids (10 mM) 0.1 mM 5 mL Penicillin-streptomycin (10,000 U/mL) 100 U/mL 5 mL L-glutamine (200 mM) 2 mM 5 mL β-mercaptoethanol (100 mM) 0.1 mM 500 μL LIF-conditioned medium (or recombinant LIF obtained commercially, e.g., Gibco, A35934) 1:500 1 mL Total N/A 501.5 mL Store at 4 ◦ C for up to 3 weeks. ..

    Recombinant:

    Article Title: Protocol for evaluating RNA-protein associations in mammalian cells with RIP-seq and RIP-qPCR.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Perl script bigsam_to_wig_mm10_wcigar2.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script scale_wiggle_rpm.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Perl script macs_strand_rand_sam.pl This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR Python script create_bkg_seq.py This study https://github.com/CalabreseLab/Protocol-forevaluating-RNA-protein-associations-in- mammalian-cells-with-RIP-seq-and-RIP-qPCR CFX Manager Bio-Rad v3.1 Prism GraphPad v9.5.0 Other Forma Series II Water-Jacketed CO 2 Incubator Thermo Fisher Scientific 3110 Vibra-Cell 130-W probe-tip sonicator Sonics VCX 130 2200 TapeStation System Agilent G2964AA High Sensitivity D1000 ScreenTape Agilent 5067–5584 High Sensitivity D1000 Reagents Agilent 5067–5585 NextSeq1000 sequencing platform Illumina 20038898 100-cycle NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit Illumina 20100987 Hard-Shell 96-Well PCR Plates Bio-Rad HSP9601 Microseal ’B’ PCR Plate Sealing Film Bio-Rad MSB1001 C1000 Touch Thermal Cycler Bio-Rad 1851196 CFX96 Real-Time System Bio-Rad 1845097 RNaseZap RNA Decontamination Solution Thermo Fisher Scientific AM9780 mESC Growth Medium Reagent Final concentration Amount DMEM (90 mL removed from 500-mL bottle before adding other reagents directly to this bottle) N/A 410 mL ESC-qualified fetal bovine serum (100%) 15% 75 mL Non-essential amino acids (10 mM) 0.1 mM 5 mL Penicillin-streptomycin (10,000 U/mL) 100 U/mL 5 mL L-glutamine (200 mM) 2 mM 5 mL β-mercaptoethanol (100 mM) 0.1 mM 500 μL LIF-conditioned medium (or recombinant LIF obtained commercially, e.g., Gibco, A35934) 1:500 1 mL Total N/A 501.5 mL Store at 4 ◦ C for up to 3 weeks. ..

    Software:

    Article Title: Age-related decline of platelet-derived growth factor receptor alpha in mesenchyme surrounding meibomian gland leads to meibomian gland dysfunction.
    Article Snippet: The meibomian glands (MGs) in the eyelids produce oil to prevent tear evaporation, a function that declines with age.. The mechanisms underlying MGs homeostasis and the pathogenesis leading to age-related meibomian gland dysfunction (ARMGD) remain largely unexplored.. MGs contain secretory acini enveloped by a substantial mesenchyme.

    SYBR Green Assay:

    Article Title: Enrichment of Hypermethylated DNA on Chip for Cervical Cancer Detection
    Article Snippet: .. Gravity flow columns, Experion DNA chips, Microseal ‘B’ PCR Plate Sealing Film, SsoAdvanced Universal SYBR Green Supermix and ddPCR 96-well plates were obtained from BioRad. .. NaCl, β-mercaptoethanol, dimethyl sulfoxide ≥99.7% (DMSO), EDTA, MgCl2 ≥ 98.0% (MgCl2), LB Broth, lysozyme from chicken egg white (≥90% lysozyme), phenylmethanesulfonyl fluoride (PMSF), ribonuclease A from bovine pancreas (RNAse), deoxyribonuclease I from bovine pancreas (DNAse), kanamycin sulfate from Streptomyces kanamyceticus, nickel(II) chloride hexahydrate (NiCl2), Nα,Nα-bis(carboxymethyl)-l-lysine hydrate ≥ 97.0%, sodium carbonate, phosphate-buffered saline (PBS) tablets for 10 mm solution, triethylamine, ammonium hydroxide solution 25%, fluorescein, H2SO4 95– 97%, 0.2 μm membrane filter, GelRed Nucleic Acid Stain, and sodium dodecyl sulfate ≥99% (SDS) were purchased from Sigma–Aldrich.

    Fluorescence:

    Article Title: Quantifying the influence of salinity and density on environmental DNA shedding and decay rates from turtles
    Article Snippet: The Bio-Rad QX200 droplet generator partitioned each reaction mixture into droplets by combining 20 μl of the reaction mixture with 70 μl of droplet generation oil, resulting in a total droplet volume of 40 μl. .. The emulsified samples were transferred to a 96-well PCR plate, heat-sealed with foil and subjected to the following thermal cycling protocol: an initial denaturation at 95°C for 5 min, followed by 40 cycles of denaturation at 95°C for 10 s and an annealing and extension step at 60°C for 30 s. After thermal cycling, the PCR plate was then transferred to the QX200 droplet reader (Bio-Rad, USA) to measure the TaqMan fluorescence of each droplet in each well. ..

    Purification:

    Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability
    Article Snippet: .. Superscript III enzyme (Thermo Fisher Scientific, Cat. No. 18080044) RNase H (New England Biolabs, Cat. No. M0297S) RNase If (New England Biolabs, Cat. No. M0243S) D1000 Screen Tape and Sample Buffer (Agilent, Cat. No. 5067-5582, 5067-5583) NEBNext Library Quant Kit for Illumina (NEB, Cat. No. E7630S) AmpureXP Purification Beads (Beckman Coulter, Cat. No. A63880) Illumina MiSeq Reagent Kit v2 (Illumina, Cat. No. MS-103-1001) PhiX Control V3 (Illumina, Cat. No. FC-110-3001) TapeStation System (Agilent, Model No. 4200) PCR plate (BIO-RAD, Cat. No. MLL9601) Adhesive PCR plate seal (BIO-RAD, Cat. No. MSB1001) Real-Time PCR (BIO-RAD, Model No. CFX Opus 96) MiSeq (Illumina, Model No. SY-410-1003) .. The library specific reverse transcription primers carry a target RNA binding region, 6-nucleotide homemade barcodes, a 4-nucleotide anchor, 10-nucleotide UMI , another 4-nucleotide anchor and part of the Illumina i7 adapter ( ).

    Control:

    Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability
    Article Snippet: .. Superscript III enzyme (Thermo Fisher Scientific, Cat. No. 18080044) RNase H (New England Biolabs, Cat. No. M0297S) RNase If (New England Biolabs, Cat. No. M0243S) D1000 Screen Tape and Sample Buffer (Agilent, Cat. No. 5067-5582, 5067-5583) NEBNext Library Quant Kit for Illumina (NEB, Cat. No. E7630S) AmpureXP Purification Beads (Beckman Coulter, Cat. No. A63880) Illumina MiSeq Reagent Kit v2 (Illumina, Cat. No. MS-103-1001) PhiX Control V3 (Illumina, Cat. No. FC-110-3001) TapeStation System (Agilent, Model No. 4200) PCR plate (BIO-RAD, Cat. No. MLL9601) Adhesive PCR plate seal (BIO-RAD, Cat. No. MSB1001) Real-Time PCR (BIO-RAD, Model No. CFX Opus 96) MiSeq (Illumina, Model No. SY-410-1003) .. The library specific reverse transcription primers carry a target RNA binding region, 6-nucleotide homemade barcodes, a 4-nucleotide anchor, 10-nucleotide UMI , another 4-nucleotide anchor and part of the Illumina i7 adapter ( ).

    Adhesive:

    Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability
    Article Snippet: .. Superscript III enzyme (Thermo Fisher Scientific, Cat. No. 18080044) RNase H (New England Biolabs, Cat. No. M0297S) RNase If (New England Biolabs, Cat. No. M0243S) D1000 Screen Tape and Sample Buffer (Agilent, Cat. No. 5067-5582, 5067-5583) NEBNext Library Quant Kit for Illumina (NEB, Cat. No. E7630S) AmpureXP Purification Beads (Beckman Coulter, Cat. No. A63880) Illumina MiSeq Reagent Kit v2 (Illumina, Cat. No. MS-103-1001) PhiX Control V3 (Illumina, Cat. No. FC-110-3001) TapeStation System (Agilent, Model No. 4200) PCR plate (BIO-RAD, Cat. No. MLL9601) Adhesive PCR plate seal (BIO-RAD, Cat. No. MSB1001) Real-Time PCR (BIO-RAD, Model No. CFX Opus 96) MiSeq (Illumina, Model No. SY-410-1003) .. The library specific reverse transcription primers carry a target RNA binding region, 6-nucleotide homemade barcodes, a 4-nucleotide anchor, 10-nucleotide UMI , another 4-nucleotide anchor and part of the Illumina i7 adapter ( ).

    Article Title: A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols from Adhesive Samplers
    Article Snippet: .. The adhesive films used as ‘Sticky Samplers’ were Microseal ‘B’ PCR Plate Sealing Films (Bio-Rad Laboratories, USA; catalogue no. MSB1001B). ..

    Real-time Polymerase Chain Reaction:

    Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability
    Article Snippet: .. Superscript III enzyme (Thermo Fisher Scientific, Cat. No. 18080044) RNase H (New England Biolabs, Cat. No. M0297S) RNase If (New England Biolabs, Cat. No. M0243S) D1000 Screen Tape and Sample Buffer (Agilent, Cat. No. 5067-5582, 5067-5583) NEBNext Library Quant Kit for Illumina (NEB, Cat. No. E7630S) AmpureXP Purification Beads (Beckman Coulter, Cat. No. A63880) Illumina MiSeq Reagent Kit v2 (Illumina, Cat. No. MS-103-1001) PhiX Control V3 (Illumina, Cat. No. FC-110-3001) TapeStation System (Agilent, Model No. 4200) PCR plate (BIO-RAD, Cat. No. MLL9601) Adhesive PCR plate seal (BIO-RAD, Cat. No. MSB1001) Real-Time PCR (BIO-RAD, Model No. CFX Opus 96) MiSeq (Illumina, Model No. SY-410-1003) .. The library specific reverse transcription primers carry a target RNA binding region, 6-nucleotide homemade barcodes, a 4-nucleotide anchor, 10-nucleotide UMI , another 4-nucleotide anchor and part of the Illumina i7 adapter ( ).

    Article Title: Discovery of tankyrase scaffolding inhibitor specifically targeting the ARC4 peptide binding domain.
    Article Snippet: .. Each condition was studied in four replicates of 20 μL and measured in a sealed MultiplateTM 96-Well PCR plate (MLL9601, Bio-Rad) with the use of a CFX96 Real-Time PCR Detection System (Bio-Rad). .. During the experiment, the temperature was gradually raised from 20 ◦ C to 95 ◦ C (1 ◦ C increase per minute) with measurements performed at intervals of 1 min. After the experiment, data were normalized and analyzed with GraphPad Prism 10 by using a nonlinear regression analysis (Boltzmann sigmoid equation).



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